Poly(acrylic acid-co-styrene), AASTY or SAA, is a highly-alternating copolymer, well-suited for the generation of native lipid nanodiscs. The reactivity ratios of styrene and acrylic acid, allow for a more alternating monomer sequence in the polymer, than 2:1 SMA copolymers.
Features - Our Individual AASTYs in comparison
Things to know in before:
- Ratio of Acrylic Acid to Styrene - 45:55, 50:50 or 55:45 | Acrylic Acid contributes to hydrophilic property of AASTY
- 5-6 kDa more aggressive; 10-11 kDa thermodynamic stable | Acrylic Acid contributes to charge
|Name||Molecular Weight||pH after solving||Divalent cationic tolerance||Acrylic Acid : Styrene Ratio|
|AASTY 6-45 ||5-6 kDa ||7.5 ||6 mM ||45% to 55% |
|AASTY 6-50 ||5-6 kDa ||7.5 ||6 mM ||50% to 50% |
|AASTY 6-55 ||5-6 kDa ||7.5 ||6 mM ||55% to 45% |
|AASTY 11-45 ||10-11 kDa ||7.5 ||6 mM ||45% to 55% |
|AASTY 11-50 ||10-11 kDa ||7.5 ||6 mM ||50% to 50% |
|AASTY 11-55 ||10-11 kDa ||7.5 ||6 mM ||55% to 45% |
AASTY is developed by Anton Autzen and Henriette Autzen, in a collaboration between the Appel Research Group at Stanford University, and the Yifan Cheng Lab at University of California San Francisco. AASTY have been found to be superior in extraction of membrane proteins compared to SMA, across multiple systems.
The exact composition of AASTY copolymers show different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs.
As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation.
Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.
We recommend the two following publications about AASTYs if you like to have more information: