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PureCube 1-Step Batch Midi Plus

Order number: 63203
PureCube 1-Step Batch Midi Plus

€70.00*

Prices plus VAT plus shipping costs

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Description

Please note that this product will be discontinued at the end of 2026.
Empty 1-step batch columns are perfectly suited for batch purifications. They can be filled with your affinity resin of choice, and allow for binding, washing, and elution in one single spin column. Designed for small to mid-scale protein purification, the PureCube 1-step batch spin columns save time and pipetting steps. Featuring SelfSeal membrane technology, the column retains resin and sample in a chamber for batch incubation. By centrifugation, the membrane pores dilate and the filtered eluate gathers in the collection chamber of the column. PureCube 1-step batch spin columns are available in two sizes: Mini for expression trials, small-scale screening, and other small-volume purification needs; or Midi Plus for volumes of up to 20 mL.

Datasheets

Features
Usage Specific binding and purification of proteins
Specifity Depending on the affinity of the used agarose resin
Binding capacity Also depending on the used agarose
Body material Polypropylene
Filter pore size 0.1 - 0.2 µm low binding PVDF
Max. volume 20 ml (midi)
Max. g force 1,500 - 2,000 xg (swing-bucket rotor)
Min. g force 100 - 200 xg for 1 - 10 min
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • 50 mL conical tubes (e.g. Falcon)
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

Citations

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