PureCube 1-Step Batch Mini
Order number: 63104
Description
Empty 1-step batch columns are perfectly suited for batch purifications. They can be filled with your affinity resin of choice, and allow for binding, washing, and elution in one single spin column. Designed for small to mid-scale protein purification, the PureCube 1-step batch spin columns save time and pipetting steps. Featuring SelfSeal membrane technology, the column retains resin and sample in a chamber for batch incubation. By centrifugation, the membrane pores dilate and the filtered eluate gathers in the collection chamber of the column. PureCube 1-step batch spin columns are available in two sizes: Mini for expression trials, small-scale screening, and other small-volume purification needs; or Midi Plus for volumes of up to 20 mL.
Datasheets
- PureCube 1-Step Batch Mini Datasheet
- Native His Affinity Purification/Detection Protocol
- Denaturing His Affinity Purification/Detection Protocol
- Native GST Affinity Purification/Detection Protocol
- Native Strep Affinity Purification/Detection Protocol
- Native Rho1D4 Affinity Purification/Detection Protocol
| Features | |
|---|---|
| Usage | Specific binding and purification of proteins |
| Specifity | Depending on the affinity of the used agarose resin |
| Binding capacity | Also depending on the used agarose |
| Body material | Polypropylene |
| Filter pore size | 0.1-0.2µm low binding PVDF |
| Max. volume | 600 µL (mini) |
| Max. g force | 12,000-14,000 x g (45-degree fixed angle rotor) |
| Min. g force | 2,500 x g for 1 min |
| Required equipment |
|