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PureCube 1-Step Batch Mini

Order number: 63104
PureCube 1-Step Batch Mini
Quantity Unit price
1
€155.00*
From 2
€138.00*

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Description

Please note that this product will be discontinued at the end of 2026.
Empty 1-step batch columns are perfectly suited for batch purifications. They can be filled with your affinity resin of choice, and allow for binding, washing, and elution in one single spin column. Designed for small to mid-scale protein purification, the PureCube 1-step batch spin columns save time and pipetting steps. Featuring SelfSeal membrane technology, the column retains resin and sample in a chamber for batch incubation. By centrifugation, the membrane pores dilate and the filtered eluate gathers in the collection chamber of the column. PureCube 1-step batch spin columns are available in two sizes: Mini for expression trials, small-scale screening, and other small-volume purification needs; or Midi Plus for volumes of up to 20 mL.

Datasheets

Features
Usage Specific binding and purification of proteins
Specifity Depending on the affinity of the used agarose resin
Binding capacity Also depending on the used agarose
Body material Polypropylene
Filter pore size 0.1-0.2µm low binding PVDF
Max. volume 600 µL (mini)
Max. g force 12,000-14,000 x g (45-degree fixed angle rotor)
Min. g force 2,500 x g for 1 min
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Microcentrifuge with rotor suitable for 2 mL centrifuge tubes (min 10,000 xg)
  • 2 mL centrifuge tubes
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment, Optional: Western Blot reagents and equipment

Citations

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