Co-NTA


Purification Resins
The term Co-NTA refers to a copper Ion that has been coupled Nitrilotriacetic acid. Co-NTA can then be coupled to agarose or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional his tagged protein. Cube Biotech offers multiple different Co-NTA based products for protein purification.

Overview of Cube Biotech's Co-NTA products for his tag protein purification.


Features

Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >30 mg/mL
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Bead Ligand Co-NTA

Co-NTA Agarose from Cube Biotech was successfully used in the following publications:

 ProteinYearAuthorProduct
eGFP 2015 Li Y., Lui Y., Gao T., Zhang B., Song Y., Terrell J-L., Barber N., Bentley W-E., Takeuchi I., Payne G-F., Wang Q.1 Co-NTA agarose (40 µm)
flavin-dependent tryptophan 6-halogenase Thal 2018  Moritzer A.-C., Minges H., Prior T., Frese M., Seewald N., Niemann H.H.2 Co-NTA agarose (40 µm)
HrpII 2017  Bauer W.S., Richardson K.A., Adams N.M., Ricks K.M., Gasperino D.J., Ghionea S.J., Rosen M., Nichols K.P., Weigl B.H., Haselton F.R., Wright D.W., 3 Co-NTA agarose (40 µm)
Metal Binding Phages 2019  Matys S., Schönberger N., Lederer F., Pollmann K.4 Co-NTA agarose (40 µm)

Different metal ions confer different binding affinity and specificity

metal_ions_protein purification affinity
Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.

References

1. Li, Y. et al. Self-assembly with orthogonal imposed stimuli to impart structure and confer magnetic function to electrodeposited hydrogel. Applied Materials & Interfaces, Published April 29, 2015
2. Moritzer, Ann-Christin et al. (2018). Structure-based switch of regioselectivity in the flavin-dependent tryptophan 6-halogenase Thal. Journal of Biological Chemistry. 294. jbc.RA118.005393. 10.1074/jbc.RA118.005393.
3. Bauer, Westley S et al. “Rapid concentration and elution of malarial antigen histidine-rich protein II using solid phase Zn(II) resin in a simple flow-through pipette tip format.” Biomicrofluidics vol. 11,3 034115. 2 Jun. 2017, doi:10.1063/1.4984788
4. Matys, S. et al. (2019). Characterization of specifically metal-binding phage clones for selective recovery of cobalt and nickel. Journal of Environmental Chemical Engineering. 103606. 10.1016/j.jece.2019.103606.
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Co-NTA
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PureCube Co-NTA Agarose PureCube Co-NTA Agarose
20 ml 50% NTA Agarose, loaded with cobalt (II) chloride
Article number: 31403
Sales price: From €107.00 *
PureCube Co-NTA MagBeads PureCube Co-NTA MagBeads
1 ml 25% NTA magnetic beads, loaded with cobalt (II) chloride
Article number: 31501
Sales price: From €68.00 *
PureCube Co-NTA Cartridge PureCube Co-NTA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with cobalt (II) chloride
Article number: 31601
Sales price: From €37.00 *
PureCube Compact Cartridge Co-NTA PureCube Compact Cartridge Co-NTA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with cobalt (II) chloride
Article number: 31602
Sales price: From €37.00 *
PentaHis antibody, BSA-free (0.1 mg) PentaHis antibody, BSA-free (0.1 mg)
Lyophilized. Manufactured by QIAGEN.  
Article number: 40040
Sales price: €448.00 *
PureCube Co-NTA Agarose XL PureCube Co-NTA Agarose XL
2 ml 50% Co-NTA Agarose with medium particle size of 400 micrometer, for special applications
Article number: 55403
Sales price: From €135.00 *
PureCube Co-NTA MagBeads XL PureCube Co-NTA MagBeads XL
1 ml 25% NTA magnetic beads, loaded with cobalt (II) chloride. Particle size 70-120 micrometer, for special applications.
Article number: 55505
Sales price: From €346.00 *
PureCube 100 Co-NTA Agarose PureCube 100 Co-NTA Agarose
2 ml 50% NTA Agarose, with 100 micron average bead size. Loaded with cobalt (II) chloride
Article number: 74403
Sales price: From €114.00 *
PureCube 100 Co-NTA Cartridge PureCube 100 Co-NTA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose with 100 micron average bead size, loaded with cobalt (II) chloride
Article number: 74601
Sales price: From €50.00 *
PureCube 100 Compact Cartridge Co-NTA PureCube 100 Compact Cartridge Co-NTA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose with 100 micron average bead size, loaded with cobalt (II) chloride
Article number: 74602
Sales price: From €50.00 *
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