His-Tag Affinity Resins & MagBeads
The polyhistidine tag is the most widely used protein affinity tag. Due to its small size of only six amino acids, it rarely interferes with the protein's function. Additionally, its low immunogenicity and versatility under native and denaturing conditions are highlighting this protein tag among others. The underlying purification principle of the His-tag is an interaction between metal ions and the imidazole ring of histidine.
