MSP1D1-His “Ultra Dark”, human
Order number: 26412
| Quantity | Unit price |
|---|---|
| 1 - 4 |
€189.00*
|
| From 5 |
€170.00*
|
Ready to ship today
Description
MSP1D1-His "Ultra Dark" is a his-tagged membrane scaffold protein (MSP) derived from apolipoprotein A-I that wraps around a phospholipid bilayer patch to form stable, water-soluble nanodiscs, enabling detergent-free solubilization and stabilization of membrane proteins in a near-native lipid environment. Its defining feature is the replacement of all tryptophan and tyrosine residues with non-fluorescent side chains, resulting in an almost non-detectable UV absorbance at 280 nm, allowing for straightforward detection and accurate concentration determination of integrated membrane proteins within the disc.
| Feature | |
|---|---|
| Form | Lyophilized |
| Amount | 2 mg |
| Available origins | Human |
| Purity | 90% (Determined by SDS-PAGE) |
| Number of amino acids | 222 |
| Molecular mass | 25.75 kDa |
| Extinction coefficient | Ext. coefficient 2980 |
| (in H2O) ε280 | Abs 0.1% (=1 g/l) 0.116 |
| Buffer | 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA |
| Affinity tag | His |
| Sequence | GHHHHHHH DYDIPTT ENLYF QG LKLLDNFDSVTSTFSKLREQL G PVTQEFFDNLEKETEGLRQEMS KDLEEVKAKVQ PFLDDFQKKFQEEMELFRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PFSDELRQRLAARLEALKENGG ARLAEFHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEFTKKLNTQ |
| Stability & Storage | Lyophilized protein: stable at -20°C for several months. Reconstituted protein: stable at 2-8°C for several days. |
| Shipping | Room temperature |
| Hazards | Product is not classified as hazardous according to (EC) No 1272/2008 [CLP].A Material Safety Data Sheet is provided. |
| Application | The reduced UV absorption at 280 nm makes MSP1D1-His "Ultra Dark" particularly advantageous over standard MSPs and alternative solubilization agents such as AASTY or SMA, enabling reliable UV-Vis based concentration determination and significantly reduced background interference in fluorescence-based assays and label-free detection methods. Beyond its spectroscopic advantages, MSP1D1-His "Ultra Dark" is well suited for Cryo-EM, ELISA, and protein-protein interaction studies where unrestricted ligand access is required, and is further compatible with cell-free expression systems and a broad range of biomedical, proteomic, and protein biochemistry applications. |
| Recommended usage | ELISA: dilute 1:100 |
| Manufacturer | Cube Biotech |
Citations
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Video
Watch our video tutorial about the creation of MSP nanodiscs. It explains the whole process, starting with the initial solubilzation using detergents. The protein that was stabilized in this demonstration was Bacteriorhodopsin.
We also recommend our video guide to nanodiscs in general.
We also recommend our video guide to nanodiscs in general.
FAQ
Can I get the datasheet for the MSP1D1-His “Ultra Dark” protein?
Is this the correct MSP product for my membrane protein?
This cannot be answered easily as every membrane protein performs differently with each nanodisc size and phospholipid. It usually requires a screening process before. Remember that membrane proteins with fewer transmembrane domains (TMD) require a smaller nanodisc, while a high number of TMDs need a larger nanodisc.
Why are there different origins for the MSP protein and do they work differently?
First, no they all work the same. Do not worry. Second, the origin of the MSP protein is of importance e.g. western blots. As the secondary antibody is usually against a species e.g anti-mouse. To avoid unwanted cross reactions the origin of the MSP protein must be known.
I have never worked with MSP nanodiscs before. Can you tell me more about them?
Of course! We created THIS MSP NANODISC GUIDE PAGE, for this purpose. Have a look at it!
Are there other options to stabilize membrane proteins besides MSP nanodiscs?
Yes, there are. We recommend synthetic copolymer nanodiscs greatly! Have a look at THIS GUIDE PAGE to learn more.
Disclaimer
For research use only
Trademark information
The owners of trademarks marked by "®" or "TM" are identified at http://www.cube-biotech.com/information/patents. Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law.
Important licensing information
This product is covered by intellectual property (IP) rights and on completion of the sale Cube Biotech grants respective Limited Use Label Licenses to purchaser. IP rights and Limited Use Label Licenses for said technology are further described and identified at http://www.cube-biotech.com/information/patents or upon inquiry at info@cube-biotech.com or at Cube Biotech GmbH, Creative-Campus-Allee 12, D-40789 Monheim, Germany. By use of this product the purchaser accepts the terms and conditions of all applicable Limited Use Label Licenses.
