Carboxy Activated


Purification Resins
Our Carboxy Activated Beads are carrying a free carboxyl group that can be activated by adding N-hydroxy-succinimide (NHS). Proteins can then bind covalently via amine groups, forming a stable peptide bond. Therefore the usuability of our Carboxy Acitvated Beads is only limited by the necessary presence of free amine groups on your biomolecule's surface.
Carboxy structural formula
Fig. 1: Structural formula of a carboxy group

Overview of Cube Biotech's Carboxy Activated Beads for biomolecule coupling via carboxy groups.

Amine Acfticated AgaroseAmine Activated MagBeads
Carboxy agarose resin (40 µm) Carboxy Activated MagBeads (30 µm)
Carboxy Activated Agarose Resin (XL sized) Carboxy Activated MagBeads (XL sized)

Features

Usage Coupling proteins to active Carboxy groups via EDC and NHS
Specifity Crosslinking reagents that contain free amine groups
Carboxy group density higher than 15 µmol/ml
Bead Ligand Affinity Bead with activated Carboxy groups



Carboxy Activated Beads by Cube Biotech were used in the following publications:
 

References

  1. Fan, M. et al. (2020). CDK13 cooperates with CDK12 to control global RNA polymerase II processivity. Science Advances. 6. eaaz5041. 10.1126/sciadv.aaz5041.
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PureCube Carboxy Agarose PureCube Carboxy Agarose
Article number: 50103
PureCube Carboxy MagBeads PureCube Carboxy MagBeads
Article number: 50201
PureCube Carboxy Agarose XL PureCube Carboxy Agarose XL
Article number: 58103
PureCube Carboxy MagBeads XL PureCube Carboxy MagBeads XL
Article number: 58201
Carboxy Activated
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PureCube Carboxy Agarose PureCube Carboxy Agarose
Cube Biotech offers Carboxy Activated Agarose resin to coat / couple biomolecules (e.g. proteins) for affinity purification.
Article number: 50103
Sales price: From €87.00 *
PureCube Carboxy MagBeads PureCube Carboxy MagBeads
Protein purification ► Carboxygroup-carrying Magnetic Beads, for activation by NHS or weak ion-exchaning ★25% conc.★
Article number: 50201
Sales price: From €85.00 *
PureCube Carboxy Agarose XL PureCube Carboxy Agarose XL
Cube Biotech offers Carboxy Activated XL Agarose resin to couple biomolecules (e.g. proteins) for affinity purification.
Article number: 58103
Sales price: From €111.00 *
PureCube Carboxy MagBeads XL PureCube Carboxy MagBeads XL
Protein purification ►Carboxy group-carrying XL Magnetic Beads, for activation by NHS or weak ion-exchaning ★25% conc.★
Article number: 58201
Sales price: From €109.00 *
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