Carboxy Activated

Our Carboxy Activated Beads are carrying a free carboxyl group that can be activated by adding N-hydroxy-succinimide (NHS). Proteins can then bind covalently via amine groups, forming a stable peptide bond. Therefore the usuability of our Carboxy Acitvated Beads is only limited by the necessary presence of free amine groups on your biomolecule's surface.

Fig. 1: Structural formula of a carboxy group

Overview of Cube Biotech's Carboxy Activated Beads for biomolecule coupling via carboxy groups.


Carboxy agarose resin (40 µm)	Carboxy Activated MagBeads (30 µm)
Carboxy Activated Agarose Resin (XL sized)	Carboxy Activated MagBeads (XL sized)

Features

Usage	Coupling proteins to active Carboxy groups via EDC and NHS
Specifity	Crosslinking reagents that contain free amine groups
Carboxy group density	higher than 15 µmol/ml
Bead Ligand	Affinity Bead with activated Carboxy groups

Carboxy Activated Beads by Cube Biotech were used in the following publications:

Protein	Year	Author	Product
Phosphopeptide Enrichment	2020	Fan. Z., Delvin J.R.m Hogg S.J., Doyle M.A., Harrison P.F., Todorovski I., Cluse L.A., Knight D.A., Sandow J.J., Gregory G., Fox A., Beilharz T.H., Kwiatkowski N., Scott N.E., Vidakovic A.T., Kelly G.P., Svejstrup J.Q., Geyer M., Gray N.S., Vervoort S.J., Johnstone R.W.¹	Carboxy Activated MagBeads
Phosphopeptide Enrichment	2020	Vervoort S., Welsh S., Delvin J.R., Barbieri E., Knight D.A., Costacurta M., Todorovski I., Kearney C.J., Sandow J.J., Bjelosevic S., Fan Z., Vissers J.H.A., Pavic K., Martin B.P., Gregary G., Kong I.Y., Hawkins E.D., Hogg S.J., Kelly M.J., Newbold A., Simpson K.J., Kauko O., Harvey K.F., Ohlmeyer M., Westermarck J., Gray N., Gardini A., Johnstone R.W.²	Carboxy MagBeads
Mass spectrometry-based proteomics	2022	Low R.R.J., Fung K.Y., Gao H., Preaudet A., Dagley F.L., Yousef J., Lee B., Larsen R.H., Kershaw N.J., Burgess A.W., Gibbs P., Hollande F., Griffin M.D.W, Grimmond S.M., Putoczki T.L³	Carboxy MagBeads

References

Fan, M. et al. (2020). CDK13 cooperates with CDK12 to control global RNA polymerase II processivity. Science Advances. 6. eaaz5041. 10.1126/sciadv.aaz5041.
Vervoort, S. et al. (2020). A PP2A-Integrator complex fine-tunes transcription by opposing CDK9. 10.1101/2020.07.12.199372.
Low R.R.J. et al. (2022). TGF-β1 induced S100 family protein expression is associated with epithelial to mesenchymal transition states and poor survival in pancreatic cancer. https://doi.org/10.1101/2022.02.25.481888

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