PlateX MP™ Strep-Tactin®XT MagBeads
Order number: 90810
Description
PlateX MP™ Strep-Tactin®XT MagBeads is a ready-to-use plate for automated membrane protein
solubilization and purification via Rho1D4-tag. It enables rapid identification of the most effective
synthetic copolymer out of eight variants for subsequent upscaling of membrane protein production.
The plate contains all required reagents - copolymers, HighSpec Rho1D4 MagBeads and
equilibration, wash and elution buffers - in a lyophilized format
We offer PlateX MP™ plates come in two plate types har - Axygen and INTEGRA, and where tested on INTEGRA and Analytic Jena instruments for which downloadable protocols exist.
We offer PlateX MP™ plates come in two plate types har - Axygen and INTEGRA, and where tested on INTEGRA and Analytic Jena instruments for which downloadable protocols exist.
Datasheets
- PlateX MP™ Protocol for CyBio FeliX by Analytic Jena
- PlateX MP™ Protocol for ASSIST-PLUS by INTEGRA
- PlateX MP™ Strep-Tactin®XT MagBeads (Axygen) Datasheet
- Technical Measurements of Axygen Plates
- PlateX MP™ Strep-Tactin®XT MagBeads (INTEGRA) Datasheet
- Copolymer / Synthetic Nanodisc MSDS (International version) + Addendum
- Copolymer / Synthetic Nanodisc MSDS (US version) + Addendum
- Strep-Tactin® MSDS (International version) + Addendum
- Strep-Tactin® MSDS (US version) + Addendum
Advantages of PlateX MP™ plates:
PlateX MP™ is a multi-component product, bringing together 5 different technologies from the Cube Biotech portfolio, and presenting multiple features.
- A time-saving pre-loaded ‘workflow mix’ for membrane protein purification that allows you to avoid long multistep experimental protocols
- Lyophilised components in the ‘workflow mix’,that ensure stability of components at room temperature
- A set of 8 NativeMP™ copolymers, that allow you to test 8 solubilisation conditions in a single run.
- Affinity Chromatography MagBeads, zhat allow purification of stabilised membrane proteins in the same well.
- Automation-ready experimental setup, including protocol for an easy set up an experiment on your liquid handling robot.
Content and setup of a PlateX MP™ plate:
| Content: | |
|---|---|
| Copolymer A | Cubipol |
| Copolymer B | Cubipol Glycerol |
| Copolymer C | Sulfo-Cubipol |
| Copolymer D | Sulfo-Cubipol Medium |
| Copolymer E | Sulfo-Cubipol Lite |
| Copolymer F | Glyco-Cubipol |
| Copolymer G | Cubipol PEG |
| Copolymer H | Cubipol Amine |
| Affinity MagBeads | MagStrep® Strep-Tactin®XT beads |
| Other Features | |
|---|---|
| Application | Automated membrane protein solubilization and purification in 96 well format with Analytik Jena CyBio Felix System or INTEGRA ASSIST PLUS System. |
| Plate type | INTEGRA 96 deep-well plate (INTEGRA, Cat. No. 6353) OR Axygen 96 deep-well plate (Corning, Cat. No. P-2ML-SQ-C) |
| Protein yield | Highly protein- and copolymer-dependent. |
| Protein Affinity | Purifies Twin-Strep-/Strep-tagged Proteins |
| Working pH | Optimal: pH 7-8 |
| Stability / Shelf-Life | 6 months after shipping |
| Storage Temperature | 4°C |
| Shipping Temperature | 4°C |
| Manufacturer | Cube Biotech |
| Hazard Information | Product is not classified as hazardous according to (EC) No 1272/2008 [CLP]. A Material Safety Data Sheet is provided. |
Citations
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Lab Results
Yield and purification performance of the gap junction protein GJB2 (29 kDa) fused to a Twin-Strep-tag®, stabilized and purified using PlateX MP™ plates. Solubilization success was evaluated by SDS-PAGE, and protein identity was confirmed by Western blot using an antibody against the Twin-Strep tag. Protein yield was quantified by intrinsic fluorescence analysis of eluates (330 nm emission; black bars) and converted to estimated concentration (grey bars) using a BSA standard curve. Black arrows indicate the target protein.
Coming soon:
Cube Biotech and Integra will soon release an Application note that will further go into the details of PlateX MP™ You will find it linked here once it is released. Planned release: February 2026
Example proteins screen and purified using PlateX MP™
| Protein | Affinity tag | Membrane protein class |
|---|---|---|
| GLP1R | Rho1D4-tag | GPCR |
| GIPR | GIPR Rho1D4-tag or FLAG-tag GPCR | GPCR |
| P2X4 | Rho1D4-tag | P2X4 Rho1D4-tag Ion Channel |
| LAMP1 | Twin-Strep-tag® | Lysosomal Glycoprotein |
| CCR5 | Twin-Strep-tag® | Receptor |
| GJB2 | Twin-Strep-tag® | Gap junction protein |
| ADORA2A | FLAG-tag | GPCR |
| GJB4 | FLAG-tag | Gap junction protein |
Disclaimer
Trademark information
The owners of trademarks marked by “®” or “TM” are identified at https://www.cube-biotech.com/information/patents. Registered names, trademarks, etc. used in this document,
even when not specifically marked as such, are not to be considered unprotected by law.
Important licensing information
This product is covered by intellectual property (IP) rights and on completion of the sale Cube Biotech grants respective Limited Use Label Licenses to purchaser. IP rights and Limited
Use Label Licenses for said technology are further described and identified at https://www.cube-biotech.com/information/patents or upon inquiry at contact@cube-biotech.com or at
Cube Biotech GmbH, Creative-Campus-Allee 12, D-40789 Monheim, Germany. By use of this product the purchaser accepts the terms and conditions of all applicable Limited Use
Label Licenses.