Nanodisc products


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Nanodiscs provide an exciting alternative for the stabilization of membrane proteins. Cube Biotech offers everything you need to get started with this novel technology. There are two options to reconstitute membrane proteins into nanodiscs:

For the reconstitution of membrane proteins that are already isolated and solubilized in detergent, we recommend to use membrane scaffold proteins (MSPs) in combination with phospholipids. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, empty nanodiscs are recommended. They can be assembled using our Nanodisc assembly kits, or purchased ready to use. Note that these kits only provide small amounts of nanodisc that are suitable for cell-free expression, and not recommended for direct reconstitution of already purified proteins.

Different nanodisc features in detail

There are several different components that a nanodisc is made from. Each with its own destinct role for the functionality of the MSP nanodisc. The following figure gives an overview of every possible composition and modification of MSP nanodiscs that we offer. In addition to the features listed in figure 1, we also offer DIBMA based synthetic Nanodiscs here.
Nanodisc differences Titleslide
 
Size:
 
MSP1D1
MSP1D1
MSP3D1
MSP2N2
 
Species:
 
Human
Mouse
Rat
Alpaca
 
Tags:
 
MSP His tagged
Untagged MSP
Biotin Labled Nanodisc
Untagged Nanodisc
 
Lipids:
 
POPC
DMPC
DMPG
Request
 
Mutations:
 
D73C
Unmutated
 
Download the nanodisc feature table.

What our customers say:

Nanodisc Janssen Testimonial
Cube Biotech ProductUsage
Lyophilized membrane scaffold proteins for reconstitution of purified proteins
Assembled nanodiscs For cell-free reactions
Assembly kits Prepare your own nanodiscs for cell-free reactions
Full nanodisc service from gene to protein Contact us! We will help you with your project.
 
Nanodisc Products
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MSP1E3D1-His, lyophilized protein MSP1E3D1-His, lyophilized protein
Membrane scaffold protein 1 E3D1, N-terminal 6xHis-tagged, 2 mg lyophilized protein  
Article number: 26152
Sales price: From €114.30 * €127.00 *
Nanodisc MSP1D1-His DMPC (50 µL) Nanodisc MSP1D1-His DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1-His protein and DMPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.  
Article number: 26311
Sales price: €369.90 * €411.00 *
mouse MSP1E3D1, lyophilized protein mouse MSP1E3D1, lyophilized protein
Membrane scaffolding protein MSP1E3D1 for nanodisc assembly. Creates scaffolding diameters of 12-14 nanometers. Lyophilized.
Article number: 26562
Sales price: From €265.00 *
PureCube DIBMA 12, Screening Kit, 10x50 mg, HEPES PureCube DIBMA 12, Screening Kit, 10x50 mg, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10X50mg | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18011
Sales price: €152.15 * €179.00 *
PureCube DIBMA 12, HEPES PureCube DIBMA 12, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18012
Sales price: From €238.85 * €281.00 *
PureCube DIBMA Mix & Match, Screening Kit, 12x50 mg, TRIS PureCube DIBMA Mix & Match, Screening Kit,...
Unsure which DIBMA size is best suited for your membrane protein experiments? Use our Mix & Match TRIS Kits to find out.
Article number: 18104
Sales price: €199.00 *
MSP1E3D1, lyophilized protein MSP1E3D1, lyophilized protein
Membrane scaffold protein 1 E3D1, His-tag removed by protease digest. 2 mg lyophilized protein
Article number: 26162
Sales price: From €243.00 *
MSP2N2, lyophilized protein MSP2N2, lyophilized protein
Membrane scaffold protein (MSP) 2N2, His tag removed by proteolytic digest. 2 mg lyophilized protein
Article number: 26182
Sales price: From €302.00 *
Nanodisc MSP1D1 dH5-His POPC (50 µL) Nanodisc MSP1D1 dH5-His POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1 deltaH5-His protein and POPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.    
Article number: 26323
Sales price: €369.90 * €411.00 *
Nanodisc MSP1E3D1-His DMPC (50 µL) Nanodisc MSP1E3D1-His DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1-His protein and DMPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.
Article number: 26351
Sales price: €369.90 * €411.00 *
Nanodisc MSP2N2 DMPG (50 µL) Nanodisc MSP2N2 DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP2N2 protein and DMPG. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26385
Sales price: €619.00 *
PureCube DIBMA 12, Screening Kit, 10x50 mg, TRIS PureCube DIBMA 12, Screening Kit, 10x50 mg, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10X50mg | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18014
Sales price: €152.15 * €179.00 *
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