Nanodisc products


PG_Logo_nanodiscs

Nanodiscs provide an exciting alternative for the stabilization of membrane proteins. Cube Biotech offers everything you need to get started with this novel technology. There are two options to reconstitute membrane proteins into nanodiscs:

For the reconstitution of membrane proteins that are already isolated and solubilized in detergent, we recommend to use membrane scaffold proteins (MSPs) in combination with phospholipids. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, empty nanodiscs are recommended. They can be assembled using our Nanodisc assembly kits, or purchased ready to use. Note that these kits only provide small amounts of nanodisc that are suitable for cell-free expression, and not recommended for direct reconstitution of already purified proteins.

Different nanodisc features in detail

There are several different components that a nanodisc is made from. Each with its own destinct role for the functionality of the MSP nanodisc. The following figure gives an overview of every possible composition and modification of MSP nanodiscs that we offer. In addition to the features listed in figure 1, we also offer DIBMA based synthetic Nanodiscs here.
Nanodisc differences Titleslide
 
     Size
MSP1D1
MSP1D1
MSP3D!
MSP2N2
 
     Species
Human
Mouse
Rat
Alpaca
 
     Tags
MSP His tagged
Untagged MSP
Biotin Labled Nanodisc
Untagged Nanodisc
 
     Lipids
POPC
DMPC
DMPG
Request
 
     Mutations
D73C
Unmutated
 
Download the nanodisc feature table.

What our customers say:

Nanodisc Janssen  Testimonial
Cube Biotech ProductUsage
Lyophilized membrane scaffold proteins for reconstitution of purified proteins
Assembled nanodiscs For cell-free reactions
Assembly kits Prepare your own nanodiscs for cell-free reactions
Full nanodisc service from gene to protein Contact us! We will help you with your project.
 
PG_Logo_nanodiscs

Nanodiscs provide an exciting alternative for the stabilization of membrane proteins. Cube Biotech offers everything you need to get started with this novel technology. There are two options to reconstitute membrane proteins into nanodiscs:

For the reconstitution of membrane proteins that are already isolated and solubilized in detergent, we recommend to use membrane scaffold proteins (MSPs) in combination with phospholipids. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, empty nanodiscs are recommended. They can be assembled using our Nanodisc assembly kits, or purchased ready to use. Note that these kits only provide small amounts of nanodisc that are suitable for cell-free expression, and not recommended for direct reconstitution of already purified proteins.

Different nanodisc features in detail

There are several different components that a nanodisc is made from. Each with its own destinct role for the functionality of the MSP nanodisc. The following figure gives an overview of every possible composition and modification of MSP nanodiscs that we offer. In addition to the features listed in figure 1, we also offer DIBMA based synthetic Nanodiscs here.
Nanodisc differences Titleslide
 
     Size
MSP1D1
MSP1D1
MSP3D!
MSP2N2
 
     Species
Human
Mouse
Rat
Alpaca
 
     Tags
MSP His tagged
Untagged MSP
Biotin Labled Nanodisc
Untagged Nanodisc
 
     Lipids
POPC
DMPC
DMPG
Request
 
     Mutations
D73C
Unmutated
 
Download the nanodisc feature table.

What our customers say:

Nanodisc Janssen  Testimonial
Cube Biotech ProductUsage
Lyophilized membrane scaffold proteins for reconstitution of purified proteins
Assembled nanodiscs For cell-free reactions
Assembly kits Prepare your own nanodiscs for cell-free reactions
Full nanodisc service from gene to protein Contact us! We will help you with your project.
 
Nanodisc Products
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MSP1E3D1-His, lyophilized protein MSP1E3D1-His, lyophilized protein
Membrane scaffold protein 1 E3D1, N-terminal 6xHis-tagged, 2 mg lyophilized protein  
Article number: 26152
Sales price: From €111.60 * €124.00 *
Nanodisc Assembly Kit MSP1D1-His_DMPC Nanodisc Assembly Kit MSP1D1-His_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, N-terminal 6xHis-tagged, 4.65 mg...
Article number: 26211
Sales price: €240.00 *
MSP2N2, lyophilized protein MSP2N2, lyophilized protein
Membrane scaffold protein (MSP) 2N2, His tag removed by proteolytic digest. 2 mg lyophilized protein
Article number: 26182
Sales price: From €296.00 *
PureCube DIBMA 12, Screening Kit, 10x50 mg, TRIS PureCube DIBMA 12, Screening Kit, 10x50 mg, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10X50mg | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18014
Sales price: €148.75 * €175.00 *
MSP1D1-His, lyophilized protein MSP1D1-His, lyophilized protein
2 mg lyophilized membrane scaffold 1D1 protein, his-tagged.
Article number: 26112
Sales price: From €111.60 * €124.00 *
MSP1D1, lyophilized protein MSP1D1, lyophilized protein
Membrane scaffold protein (MSP)1D1, His tag removed by proteolytic digest. 2 mg lyophilized protein
Article number: 26132
Sales price: From €238.00 *
Nanodisc MSP1D1-His_DMPC (50 µL) Nanodisc MSP1D1-His_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1-His protein and DMPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.  
Article number: 26311
Sales price: €361.80 * €402.00 *
PureCube DIBMA 10, 1g, TRIS PureCube DIBMA 10, 1g, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=10.000 g/mol
Article number: 18005
Sales price: From €233.75 * €275.00 *
MSP1D1 dH5-His, lyophilized protein MSP1D1 dH5-His, lyophilized protein
Membrane scaffold protein 1 D1 delta H5 mutant, N-terminal 6xHis-tagged, 2 mg lyophilized protein
Article number: 26122
Sales price: From €111.60 * €124.00 *
Nanodisc Assembly Kit MSP1E3D1-His_DMPC Nanodisc Assembly Kit MSP1E3D1-His_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1E3, N-terminal 6xHis-tagged, 5.09 mg...
Article number: 26251
Sales price: €240.00 *
Nanodisc MSP1D1_POPC (50 µL) Nanodisc MSP1D1_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1 protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26333
Sales price: €526.00 *
Nanodisc MSP2N2-His_DMPC (50 µL) Nanodisc MSP2N2-His_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP2N2-His protein and DMPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.
Article number: 26371
Sales price: €443.70 * €493.00 *
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