Nanodisc products

For the reconstitution of membrane proteins that are already isolated and solubilized in detergent, we recommend to use membrane scaffold proteins (MSPs) in combination with phospholipids. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, empty nanodiscs are recommended. They can be assembled using our Nanodisc assembly kits, or purchased ready to use. Note that these kits only provide small amounts of nanodisc that are suitable for cell-free expression, and not recommended for direct reconstitution of already purified proteins.

What our customers say:

Cube Biotech Product	Usage
Lyophilized membrane scaffold proteins	for reconstitution of purified proteins
Assembled nanodiscs	For cell-free reactions
Assembly kits	Prepare your own nanodiscs for cell-free reactions
Full nanodisc service from gene to protein	Contact us! We will help you with your project.