Nanodisc products


PG_Logo_nanodiscs

Nanodiscs provide an exciting alternative for the stabilization of membrane proteins. Cube Biotech offers everything you need to get started with this novel technology. There are two options to reconstitute membrane proteins into nanodiscs:

For the reconstitution of membrane proteins that are already isolated and solubilized in detergent, we recommend to use membrane scaffold proteins (MSPs) in combination with phospholipids. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a His-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, empty nanodiscs are recommended. They can be assembled using our Nanodisc assembly kits, or purchased ready to use. Note that these kits only provide small amounts of nanodisc that are suitable for cell-free expression, and not recommended for direct reconstitution of already purified proteins.

Different nanodisc features in detail

There are several different components that a nanodisc is made from. Each with its own destinct role for the functionality of the MSP nanodisc. The following figure gives an overview of every possible composition and modification of MSP nanodiscs that we offer. In addition to the features listed in figure 1, we also offer DIBMA based synthetic Nanodiscs here.
Nanodisc differences Titleslide
 
Size:
 
MSP1D1
MSP1D1
MSP3D1
MSP2N2
 
Species:
 
Human
Mouse
Rat
Alpaca
 
Tags:
 
MSP His tagged
Untagged MSP
Biotin Labled Nanodisc
Untagged Nanodisc
 
Lipids:
 
POPC
DMPC
DMPG
Request
 
Mutations:
 
D73C
Unmutated
 
Download the nanodisc feature table.

What our customers say:

Nanodisc Janssen Testimonial
Cube Biotech ProductUsage
Lyophilized membrane scaffold proteins for reconstitution of purified proteins
Assembled nanodiscs For cell-free reactions
Assembly kits Prepare your own nanodiscs for cell-free reactions
Full nanodisc service from gene to protein Contact us! We will help you with your project.
 
Nanodisc Products
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MSP1E3D1-His, lyophilized protein MSP1E3D1-His, lyophilized protein
Membrane scaffold protein 1 E3D1, N-terminal 6xHis-tagged, 2 mg lyophilized protein  
Article number: 26152
Sales price: From €131.00 *
Synthetic Nanodisc Screening Kit, (8x2x50 mg) Synthetic Nanodisc Screening Kit, (8x2x50 mg)
Article number: 18290
Sales price: €320.00 *
MSP2N2-His, lyophilized protein MSP2N2-His, lyophilized protein
Membrane scaffold protein 2 N2, N-terminal 6xHis-tagged, 2 mg lyophilized protein
Article number: 26172
Sales price: From €164.00 *
SMALP Screening Set (12x50 mg) SMALP Screening Set (12x50 mg)
Article number: 18280
Sales price: €258.00 *
Nanodisc MSP1D1-His DMPC (50 µL) Nanodisc MSP1D1-His DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1-His protein and DMPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.  
Article number: 26311
Sales price: €424.00 *
DIBMA Screening Kit, TRIS (12x50 mg) DIBMA Screening Kit, TRIS (12x50 mg)
Unsure which DIBMA is best suited for your membrane protein experiments? Use our TRIS Screening Set to find out.
Article number: 18104
Sales price: €205.00 *
SMALP 25010S SMALP 25010S
Our SMALP polymer products are made to provide an easy way to stabilize and solubilize membrane proteins.
Article number: 18200
Sales price: From €185.00 *
DIBMA Screening Kit, HEPES (12x50 mg) DIBMA Screening Kit, HEPES (12x50 mg)
Unsure which DIBMA is best suited for your membrane protein experiments? Use our DIBMA HEPES Screening Set to find out.
Article number: 18101
Sales price: €205.00 *
DIBMA 10, HEPES DIBMA 10, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10x50mg | Buffered in HEPES. | MW=10.000 g/mol
Article number: 18001
Sales price: From €185.00 *
MSP2N2, lyophilized protein MSP2N2, lyophilized protein
Membrane scaffold protein (MSP) 2N2, His-tag removed by proteolytic digest. 2 mg lyophilized protein
Article number: 26182
Sales price: From €312.00 *
MSP1D1-His, lyophilized protein MSP1D1-His, lyophilized protein
2 mg lyophilized membrane scaffold 1D1 protein, His-tagged.
Article number: 26112
Sales price: From €131.00 *
Nanodisc MSP1E3D1-His POPC (50 µL) Nanodisc MSP1E3D1-His POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1-His protein and POPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.  
Article number: 26353
Sales price: €424.00 *
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